山羊CC类趋化因子受体（CCR2）基因的克隆及组织表达分析

doi:10.11843/j.issn.0366-6964.2016.11.007

Abstract

Abstract:

The aim of the study was to clone the CDS of CCR2 gene, and to determine its expression in various tissues, which might provide basis for its functional research on regulating lipid metabolism. Each of 4 healthy, 2-3 years old castrated Jianzhou Big-eared goats and Tibetan goats were selected. After slaughter, the tissue samples from heart, liver, spleen, lung, kidney, longissimus dorsi muscle, biceps femoris muscle, triceps brachii muscle and subcutaneous fat were collected for the total protein and RNA extraction. The sequence of CCR2 gene was cloned by RT-PCR and analyzed using bioinformatics. The mRNA and protein expression of CCR2 was determined using real-time fluorescent quantitative (qPCR) and Western blot, respectively. A length of 1 186 bp cDNA of CCR2 gene was cloned, containing 1 100 bp CDS, 2 bp 5′ UTR and 74 bp 3′ UTR, encoding 370 amino acids. The goat CCR2 protein shared 97.3%, 91.9%, 77.0% and 69.2% similarity with bovine, pig, mouse and human, respectively. The goat CCR2 gene was closest associated with that of bovine, followed by pig, dog and mouse, and distant with human. The tissue expression analysis showed that expression of CCR2 was higher in kidney and heart, and lowest in subcutaneous fat and muscles in both of the goat breeds. These data indicate that CCR2 gene may play an important role in regulating lipid metabolism in goat.

CLC Number:

S827
S813.3

ZHU Jiang-jiang, LIN Ya-qiu, WANG Yong, LI Qian, LIN Sen. Molecular Cloning and Tissue Expression of Goat Chemokine (CC motif) Receptor 2 (CCR2) Gene[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(11): 2202-2209.

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Molecular Cloning and Tissue Expression of Goat Chemokine (CC motif) Receptor 2 (CCR2) Gene

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